Assessment of five phenotypic tests for detection of methicillin-resistant staphylococci in Cotonou, Benin
| Autor: | Séverin Anagonou, Achille Massougbodji, A.P. Wachinou, Lucrece Ahovegbe, Wilfried Bekou, Frank Faïhun, Frédéric Sogbo, Aderemi Kehinde, Dissou Affolabi, Mathieu Odoun |
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| Rok vydání: | 2014 |
| Předmět: |
business.industry
SCCmec Plant Science biochemical phenomena metabolism and nutrition medicine.disease_cause Microbiology Methicillin resistance law.invention Mueller-Hinton agar chemistry.chemical_compound Infectious Diseases chemistry law Direct agglutination test polycyclic compounds medicine bacteria Cefoxitin business Staphylococcus Polymerase chain reaction medicine.drug |
| Zdroj: | African Journal of Microbiology Research. 8:1112-1117 |
| ISSN: | 1996-0808 |
| DOI: | 10.5897/ajmr2014.6604 |
| Popis: | The study aimed at assessing performance and cost of phenotypic tests for detecting methicillin resistance in Staphylococcus spp. isolates in Cotonou, Benin. Isolates consecutively collected from various specimens from four medical laboratories in Cotonou from December 2012 to April 2013 were included in the study. The isolates were subjected to five phenotypic tests: disk diffusion tests with cefoxitin (Cefox) and moxalactam (Moxa) on Mueller Hinton agar incubated at 37°C, oxacillin on Mueller Hinton agar incubated at 30°C (Oxa30), oxacillin on salt Mueller Hinton agar incubated at 37°C (Oxa37) and agglutination test for PBP2a detection (TPBP 2a). Results were compared with polymerase chain reaction (PCR) of mecA gene which was used as the gold standard. In addition, cost per reagent of each phenotypic test was assessed. Considering the general agreement with PCR, Cefox and Moxa were the best tests in S. aureus while in non-aureus Staphylococcus isolates, TPBP 2a was the best test but its cost was 20 times higher than that of disk diffusion tests. Key words: Staphylococcus spp., methicillin resistance, diffusion disk tests, PBP 2a, mecA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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