Allele-specific polymerase chain reaction
| Autor: | Luis A. Ugozzoli, Bijay K. Pal, Dan Y. Wu, R. Bruce Wallace |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Genetics
Inverse polymerase chain reaction Hybridization probe Biology Molecular biology General Biochemistry Genetics and Molecular Biology law.invention Restriction enzyme law Multiplex polymerase chain reaction Primer (molecular biology) Allele Molecular Biology Nested polymerase chain reaction Polymerase chain reaction |
| Zdroj: | Methods. 2:42-48 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/s1046-2023(05)80124-0 |
| Popis: | A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one allele and a mismatch with the other allele. This method allows the direct detection of the normal or the sickle cell β-globin allele in genomic DNA without the additional steps of probe hybridization, ligation or restriction enzyme cleavage. |
| Databáze: | OpenAIRE |
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