Isolation and characterization of galactomannans from Prosopis affinis as potential gum substitutes
| Autor: | Guillermo Moyna, Eliana Budelli, Zohra Bennadji, Fernando Ferreira, Pilar Vilaró, Luis Alberto Panizzolo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Guar gum Chromatography biology 010405 organic chemistry General Chemical Engineering Intrinsic viscosity Chemical structure 02 engineering and technology General Chemistry Food chemistry 021001 nanoscience & nanotechnology Polysaccharide biology.organism_classification 01 natural sciences 0104 chemical sciences Galactomannan chemistry.chemical_compound chemistry Monosaccharide 0210 nano-technology Prosopis affinis Food Science |
| Zdroj: | Food Hydrocolloids. 77:711-719 |
| ISSN: | 0268-005X |
| DOI: | 10.1016/j.foodhyd.2017.10.038 |
| Popis: | Galactomannans from the endosperm of mature seeds of Prosopis affinis were isolated following different extraction procedures in yields of up to 64%. The polysaccharides were characterized by HPLC, GC-MS, and NMR spectroscopy, and their physicochemical properties were studied by SEC/MALLS-QUELS/RI and rheological methods. The monosaccharide composition of the extracted galactomannans showed only mannose and galactose in a ∼1.5 ratio, similar to galactomannans found in guar gum and obtained from other Prosopis species. The structural characterization showed that these polysaccharides are composed by a β-(1→4)-linked mannose backbone with galactopyranosyl residues attached through α-(1→6) linkages. The weight-average molecular weight (Mw) of the P. affinis galactomannans went from (1.6 ± 0.2) x 106 to (1.8 ± 0.1) x 106 g/mol, and the intrinsic viscosity, in the 9.9 to 10.7 dL/g range, was comparable to that reported for other extracts from the Prosopis genre. Although the molecular weight distribution and intrinsic viscosity were dependent on the extraction procedure, the mannose-to-galactose ratio was almost invariant. Conformation plots showed that the polymer extracted at higher temperatures is more cohesive, a characteristic that could be attributed to a greater number of polymer-polymer interactions. Our findings show that the P. affinis galactomannan has nearly monomodal molecular weight distribution, functional properties similar to those of guar gum, and can be isolated from an abundant and readily available non-traditional source. It therefore represents a promising new biomaterial for the food and pharmaceutical industries that can be produced from the regional native flora in a sustainable way. |
| Databáze: | OpenAIRE |
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