| Popis: |
Nicotine is a class of alkaloids that are found in plants with the family name Solanaceae, such as tobacco (Nicotiana). This plant is widely used as a raw material for making cigarettes. The separation of nicotine in the urine of active smokers has a very important role in forensic chemistry. A purification technique using column chromatography was developed to separate impurities from the extracted nicotine. Moringa leaves have high adsorption properties so that they can be used as a filler in chromatography columns. This study aims to separate nicotine from the urine of active smokers using Moringa leaves as a filler in the chromatography column. Experimental methods have been developed in this work. The preparation and extraction process begins with collecting Moringa leaves, cleaning, drying, then activating at 5000C for 4 hours. The urine preparation of active smokers is done by taking 50 ml of urine and then adding 50 ml of chloroform solvent. The process of separating nicotine from urine was carried out by column chromatography using active moringa leaves as fillers in the chromatography column as much as 10 g, 20 g, 30 g, 40 g, and 50 g. The separation was followed by maceration of the electrosynthetic coupling for 120 minutes for further separation. The pH is maintained at 9 in order to obtain optimal results. The extracted nicotine was tested using Cyanogen bromide to produce an orange precipitate, paper chromatography was developed and produced Rf values in the range 0.45 - 0.46. Nicotine analysis was continued using UV spectroscopy and resulted in nicotine levels in the variation of 10 g, 20 g, 30 g, 40 g, and 50 g moringa leaves, respectively 0.55 ppm, 1.75 ppm, 3.82 ppm, 3.34 ppm, and 2.59 ppm. |
