Autohydrolysis and microwave ionic liquid pretreatment of Pinus radiata: Imaging visualization and analysis to understand enzymatic digestibility
| Autor: | Sandra Rivas, Francisco Rodríguez, Mercedes Oliet, Tamara M. Santos, M. Virginia Alonso, Victoria Rigual, Juan Carlos Domínguez |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Chromatography Softwood 010405 organic chemistry Fractionation 01 natural sciences 0104 chemical sciences Hydrolysis chemistry.chemical_compound chemistry Enzymatic hydrolysis Ionic liquid Lignin Agronomy and Crop Science Chemical composition 010606 plant biology & botany Glucan |
| Zdroj: | Industrial Crops and Products. 134:328-337 |
| ISSN: | 0926-6690 |
| Popis: | Sequential pretreatments provide advantages of every single process towards a complete biomass fractionation. In this work, autohydrolysis and IL microwave pretreatments are sequentialy studied, and their effect to enhance enzymatic hydrolysis is analyzed. Mild, intermediate and severe autohydrolysis are combined with four IL temperatures (50, 80, 120 and 150 °C). Pretreated solids are enzymatically hydrolyzed and compared in terms of chemical composition and morphology. Digital image analysis is employed to numerically determine the heterogeneity of the solids using surface fractal dimension and lacunarity parameters. In this study, the negative effect of intermediate and severe autohydrolysis, over the subsequent IL pretreatment is demonstrated. Mild autohydrolysis and high IL conditions (AH150IL120) results in digestibilities of 78.8 g of glucan/100 g of glucan introduced. High surface fractal values (in the range of 2.5461–2.7124) and low lacunarities (0.0818–0.2563) enhance the enzymatic accessibility of pine wood. Furthermore, the negative effect of softwood lignin accumulation in the surface is observed using confocal fluorescence microscopy. |
| Databáze: | OpenAIRE |
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