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Background: Blastocystis is a typical anaerobic colon protist in humans with controversial pathogenicity and has relation with alterations in the intestinal microbiota composition (dysbiosis), whose indicator is the Firmicutes/Bacteroidetes ratio (F/B ratio); this indicator is also linked to complications such as diabetes, obesity, or inflammatory bowel disease. The present study investigated the prevalence of Blastocystis and its association with intestinal dysbiosis in healthy and metabolic diseased subjects.Methods: Fecal and blood samples were collected consecutively from 200 healthy subjects, and 84 with diseased metabolic subjects; Blastocystis and its most frequent subtypes were identified by end-point PCR and the two most representative phyla of the intestinal microbiota Firmicutes and Bacteroidetes by real-time PCR.Results: The prevalence of Blastocystis in healthy subjects was 47.0%, and 65.48 % in subjects with metabolic disease; the most prevalent subtype in the total population was ST3 (28.38 %), followed by ST1 (14.86 %), ST4, ST5, and ST7 (each one of them with 14.19 % respectively), and finally ST2 (8.78 %). The low F/B ratio that characterizes intestinal dysbiosis was associated with the prevalence of Blastocystis in the two cohorts FACSA (OR = 3.78 P p Conclusions: The predatory role of Blastocystis over Firmicutes phylum is evident in both cohorts since it was observed that the abundance of the beneficial bacterial group's Bacteroidetes increases in the groups colonized by this eukaryote and, therefore, may have a beneficial effect. |
