Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
Autor: | Promjit Saralamp, Y. Phaidee, Sompop Prathanturarug, Wongsatit Chuakul, Noppamas Soonthornchareonnon |
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Rok vydání: | 2005 |
Předmět: | |
Zdroj: | Plant Cell, Tissue and Organ Culture. 80:347-351 |
ISSN: | 1573-5044 0167-6857 |
DOI: | 10.1007/s11240-004-1020-x |
Popis: | Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production. |
Databáze: | OpenAIRE |
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