Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

Autor: Promjit Saralamp, Y. Phaidee, Sompop Prathanturarug, Wongsatit Chuakul, Noppamas Soonthornchareonnon
Rok vydání: 2005
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture. 80:347-351
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-004-1020-x
Popis: Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.
Databáze: OpenAIRE