| Autor: |
J. Zhou, D. Doueck, R. R. Ladi, Bharat Madan, Jacy R. Wolfe, N. Boyle, C. Yu Chung, David Price, S. N. Lopez Acevedo, Sian Llewellyn-Lacey, Rodrigo Matus-Nicodemos, A. S. Fahad, Amy D. Laflin, H. H. Balfour, Brandon J. DeKosky, Matias Gutiérrez-González |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Functional analyses of the T cell receptor (TCR) landscape can reveal critical information about protection from disease and molecular responses to vaccines. However, it has proven difficult to combine advanced next-generation sequencing technologies with methods to decode the peptide-major histocompatibility complex (pMHC) specificity of individual TCRs. Here we developed a new high-throughput approach to enable repertoire-scale functional evaluations of natively paired TCRs. In particular, we leveraged the immortalized nature of physically linked TCRα:β amplicon libraries to analyze binding against multiple recombinant pMHCs on a repertoire scale. To exemplify the utility of this approach, we also performed affinity-based functional mapping in conjunction with quantitative next-generation sequencing to track antigen-specific TCRs. These data successfully validated a new immortalization and screening platform to facilitate detailed molecular analyses of human TCRs against diverse antigen targets associated with health, vaccination, or disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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