A simple and rapid method for identification and determination of cordycepin in Cordyceps militaris by capillary electrophoresis
| Autor: | Chia-Hsien Chou, Yew-Min Tzeng, Yerra Koteswara Rao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chromatography
biology Cordycepin Chemistry Metabolite biology.organism_classification Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Cordyceps militaris Environmental Chemistry Sample preparation Methanol Spectroscopy Active metabolite |
| Zdroj: | Analytica Chimica Acta. 566:253-258 |
| ISSN: | 0003-2670 |
| Popis: | Cordycepin is the main active metabolite of Cordyceps militaris extracts; according to recent studies it has interesting therapeutic activities. A new capillary electrophoresis (CE) procedure with UV detection at 254 nm for determination of cordycepin was developed and optimized. Optimal conditions found were 20 mM sodium borate buffer with 28.6% methanol, pH 9.5, separation voltage 20 kV, hydrodynamic injection time 10 s and temperature 25 °C. Linearity was found over the 20–100 μg/mL concentration ranges of cordycepin. The developed method has been applied for determination of cordycepin in various pharmaceutical products. A comparison was made between CE and a high performance liquid chromatography (HPLC) method. Both of these methods gave comparable results. The shorter analysis time and low running cost are the main advantages of CE method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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