Mumia quercus sp. nov., isolated from the root of Quercus variabilis
| Autor: | Hongmin Chen, Tingting Cao, Teng Wang, Congting Gao, Jingquan Yang, Qingyan Dong, Yue Shen, Chongxi Liu |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 73 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.005776 |
| Popis: | A novel endophytic actinomycete, designated strain NEAU-365T, was isolated from the root of Quercus variabilis collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365T belonged to the genus Mumia but clearly differs from the currently recognized species Mumia zhuanghuii Z350T (99.31 %), Mumia xiangluensis NEAU-KD1T (98.82 %) and Mumia flava MUSC 201T (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365T clustered with the type strains of the genus Mumia . The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365T and those of M. zhuanghuii Z350T(27.5 and 84.0 %), M. xiangluensis NEAU-KD1T(23.4 and 80.9 %) and M. flava MUSC 201T(20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained ll-diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H4). The major fatty acids were C16 : 0, C18 : 0, C18 : 1 ω9c and 10-methyl C18 : 0. On the basis of genotypic and phenotypic differences from members of the genus Mumia , a novel species, Mumia quercus sp. nov. is proposed. The type strain is NEAU-365T (=CCTCC AA 2021033T=JCM 35005T). |
| Databáze: | OpenAIRE |
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