On the desalting of solutions of amino acids by ion exchange

Autor:	Stanford Moore, Edouard-Jean Bigwood, Albert Dreze
Rok vydání:	1954
Předmět:	chemistry.chemical_classification Chromatography Ion exchange Elution Tryptophan Salt (chemistry) Biochemistry Analytical Chemistry Amino acid Acetic acid chemistry.chemical_compound Paper chromatography chemistry Anion-exchange chromatography Environmental Chemistry Spectroscopy
Zdroj:	Analytica Chimica Acta. 11:554-558
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(00)87789-2
Popis:	The removal of sodium citrate and phosphate buffers from amino acids in the effluent from ion exchange chromatograms can be accomplished by one-step desalting on basic or acidic resins. At “salt/amino acid” ratios of 1000 to 1, the amino acid recovery is quantitative. The neutral and acidic amino acids are adsorbed on a strong base resin (Dowex 2) and eluted with acetic acid. The basic amino acids and tryptophan are adsorbed on an acid resin (Dowex 50) and eluted with HCl. The resulting salt-free solutions are suitable for examination by paper chromatography or for the isolation of amino acids for further characterization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::89e1c5f57620a814b27059b510de7c68 https://doi.org/10.1016/s0003-2670(00)87789-2 Zobrazit plný text záznamu