The Role of Human Herpesvirus 8 Molecular Characterization in the Management of HIV Infected Patients Diagnosed with Malignancies Associated with Its Infection

Autor:	Martínez Pedro Ariel, Ulrich Hengge, Ugarte Yaumara, Caballero Iraida, Capó . Virginia, Blanco González Orestes, Jiménez . Narciso, Fleites Gilberto, Kourí Cardellá Vivian, Álvarez . Alina, Alemán Campos Yoan, Abad Yoandra, Calderón Odalys, Verdasquera Denis
Rok vydání:	2013
Předmět:	Pleural effusion viruses virus diseases Lymphoproliferative disorders biochemical phenomena metabolism and nutrition Biology medicine.disease Virology Lymphoma Acquired immunodeficiency syndrome (AIDS) hemic and lymphatic diseases Immunology medicine Sarcoma B-cell lymphoma Viral load Genotyping
Zdroj:	World Journal of AIDS. :221-230
ISSN:	2160-8822 2160-8814
DOI:	10.4236/wja.2013.33030
Popis:	Despite the progress has been reached with Human herpesvirus 8 (HHV-8) research, there are gaps in the knowledge of viral induced oncogenesis. The aim of the present study was to identify possible associations between HHV-8 subtypes, HHV-8 loads and clinical manifestations of HIV infected patients diagnosed with different malignancies associated with HHV-8 infection. Forty six HIV-1 infected individuals diagnosed with different HHV-8 associated diseases were studied [37 epidemic Kaposi’s sarcoma (KS), 3 pleural effusion lymphoma (PEL); 5 peripheral lymphadenopathies (PL); 1 Hodgkin’s lymphoma (HL); 1 non Hodgkin’s lymphoma (NHL)]. HHV-8 loads were determined by quantitative real time PCR (qRT-PCR) whilst HHV-8 subtypes were determined by open-reading frame (ORF)-K1 gen genotyping. HHV-8 subtypes B, A, C, A5 and E were exhibited by 31.8%, 23.4%, 19.1%, 17% and 8.5% of the studied patients, respectively. The median HHV-8 viral load did not differ between subtypes (p > 0.05) but HHV-8 viral loads were significantly higher in PEL than in epidemic KS lesion or lymph nodes (p = 0.04). Subtype B was detected in 60% of patients with B cell lymphoma (NHL, PEL and HL) whereas subtype E was only detected in patients with epidemic KS diagnosis. Our data suggest that HHV-8 DNA quantification instead of subtype identification could be used as a surrogate marker for monitoring its infection, not only in epidemic KS patients but also in HIV infected individuals with lymphoproliferative disorders.
Databáze:	OpenAIRE
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