Ferricyanide restores nitrate reductase activity in leaf extracts of water stressed wheat seedlings

Autor:	Veena Sawhney, Surinder K. Sawhney, Neeru Munjal
Rok vydání:	1998
Předmět:	chemistry.chemical_classification Physiology Plant Science Reductase Biology Nitrate reductase Enzyme assay chemistry.chemical_compound Potassium ferricyanide Horticulture Enzyme chemistry Biochemistry biology.protein Poaceae Ferricyanide Agronomy and Crop Science Incubation
Zdroj:	Journal of Plant Physiology. 152:577-579
ISSN:	0176-1617
DOI:	10.1016/s0176-1617(98)80280-0
Popis:	Summary Nitrate reductase (EC 1.6.6.1) activity declined rapidly in leaves of wheat seedlings following polyethylene glycol-induced water stress. The decrease in NADH-nitrate reductase was accompanied by a concomitant loss of FMNH 2 − and reduced benzyl viologen-linked nitrate reductase activities. Incubation of dialysed leaf extracts with potassium ferricyanide restored the enzyme activity almost to the same level as that in non-stressed plants. The decline in nitrate reductase, at least during the initial stages of water stress, hence seems to be primarily due to redox-mediated inactivation rather than diminished synthesis of the enzyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::89d146e1ab4d94637c0081cc5c6bd433 https://doi.org/10.1016/s0176-1617(98)80280-0 Zobrazit plný text záznamu Full Text from ScienceDirect