Quick detection of cell to cell communication in gram negative bacteria by colour change of polymer matrix entrapping reporter bacteria
| Autor: | Eri Nasuno, Chigusa Okano, Norihiro Kato, Ken-ichi Iimura, Tomohiro Morohoshi, Tsukasa Ikeda |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Gram-negative bacteria biology Mechanical Engineering food and beverages biochemical phenomena metabolism and nutrition Matrix (biology) Condensed Matter Physics biology.organism_classification Absorbance Quorum sensing Pigment Biochemistry Mechanics of Materials visual_art visual_art.visual_art_medium Bioassay General Materials Science Chromobacterium violaceum Bacteria |
| Zdroj: | Materials Research Innovations. 18:S4-879 |
| ISSN: | 1433-075X 1432-8917 |
| DOI: | 10.1179/1432891714z.000000000795 |
| Popis: | Quick and easy bioassay system for detection of N-acyl-L-homoserine lactones (AHLs) became possible by determining colour change of polymer matrix surfaces entrapping Chromobacterium violaceum CV026 cells, which accumulates purple pigment violacein inside cells through cell–cell communication system termed as quorum sensing (QS). The AHL is popularly produced as one of the QS signals to regulate various cell functions, and its concentration increase can lead to activate the QS. Sensitive detection of producing AHLs could be realised on the polymer surfaces as the gradual changing purple because the AHL synthase defective strain CV026 could respond to produce purple violacein in the presence of AHLs derived from other bacteria. The b* coordinate representing the difference between yellow and blue chromas in the CIE-L*a*b* colour space was obviously negatively correlated with absorbance at 585 nm of butanol extracted violacein from cells irrespective of culture time and initial concentration of cell... |
| Databáze: | OpenAIRE |
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