Fragment DNA as vector for genetic transformation of bean (Phaseolus vulgaris L.)

Autor:	Francisco J. L. Aragão, Giovanni R. Vianna, Margareth M. C. Albino, Elíbio L. Rech, Bárbara B. A. Dias, Lı́lian de Mesquita Silva
Rok vydání:	2004
Předmět:	Genetics Genetic transfer food and beverages Genetically modified crops Horticulture Biology biology.organism_classification chemistry.chemical_compound Transformation (genetics) Plasmid chemistry Phaseolus Gene DNA Southern blot
Zdroj:	Scientia Horticulturae. 99:371-378
ISSN:	0304-4238
DOI:	10.1016/s0304-4238(03)00107-9
Popis:	The aim of this study was verify the use of fragment DNA as vector for genetic transformation of bean in order to replace circular vectors containing antibiotic resistance genes. The 1.5 kb DNA fragment carrying the bar gene was used to generate transgenic bean plants through microparticles bombardment. The frequency of bean transformation achieved was compared with transformation using the entire circular plasmid. The results have demonstrated that the transformation frequencies were similar. For the variety Carioca the frequencies were 0.8% (using DNA fragment) and 0.7% (using circular vector). For the variety Olathe the frequencies were 0.2% (using DNA fragment) and 0.3% (using circular vector). In addition, Southern blot analyses revealed similar pattern complexity in transgenic plants obtained either with the entire plasmid or DNA fragment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::89c64e9f70f153d32edc68f24fc67196 https://doi.org/10.1016/s0304-4238(03)00107-9 Zobrazit plný text záznamu Full Text from ScienceDirect