Primary cell culture from pig neonatal thyroid gland: Growth, folliculogenesis, and hormone activity

Autor: T. P. Bondarenko, Yu. V. Malyukin, G. A. Bozhok, E. I. Legach, S. B. Bilyavskaya, I. A. Borovoy, I. M. Gella
Rok vydání: 2013
Předmět:
Zdroj: Cell and Tissue Biology. 7:512-521
ISSN: 1990-5203
1990-519X
Popis: A comprehensive assay of proliferative and hormonal activity in primary cell cultures derived from neonatal pig thyroid was carried out for the first time. Morphology and basal and thyroid-stimulating hormone (TSH)-stimulated secretion of thyroxin were evaluated in cultures, depending on the initial material placed into the culture: single cells or follicular conglomerates. Spontaneous and under chronic TSH stimulation folliculogenesis and formation of dome structures were assessed in culture. It was demonstrated that the cells expressed β-III-tubulin during prolonged cultivation with nerve growth factor.
Databáze: OpenAIRE