STUDY OF VIRULENCE FACTORS IN UROPATHOGENIC ESCHERICHIA COLI
| Autor: | J. Vijayalakshmi, R. Hymavathi, M. V. Ramanamma, V. Anitha Lavanya, G. Swarnalatha, R. Somasekhar, A. Surekha, A. Renuka Devi, V. Anitha |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Evolution of Medical and Dental Sciences. :1297-1305 |
| ISSN: | 2278-4802 2278-4748 |
| DOI: | 10.14260/jemds/2015/182 |
| Popis: | Uropathogenic E. coli (UPEC) is a causative agent in the vast majority of Urinary Tract infections (UTIs), including Cystitis, Pyelonephritis which may result in renal failure in healthy individuals and in renal transplant patients. UPEC express a multitude of virulence factors to break the inertia of the mucosal barrier. PURPOSE: To study the virulence factors and antimicrobial susceptibility pattern. METHODS: 200 E.coli strains from symptomatic cases of UTI and 50 E.coli strains from other clinical samples were taken as controls. Samples were screened for virulence factors like Haemolysin, Mannose resistant, Mannose sensitive Haemagglutination (MRHA, MSHA), Serum resistance and Cell surface hydrophobicity (CSH) by recommended methods. Antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method as per CLSI guidelines. RESULTS: Among 200 E.coli strains 50(25%) were haemolytic, 60(30%) showed MRHA, 76(38%) showed MSHA, 98(49%) were serum resistant and 86(43%) were hydrophobic. Among 50 controls 8 (16%) were haemolytic, 2(4%) showed MRHA, 4(8%) showed MSHA, 16(32%) were serum resistant and 3(6%) were hydrophobic. The difference between cases and controls for MRHA, Serum resistance, Cell surface hydrophobicity production were significant (p |
| Databáze: | OpenAIRE |
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