Microwell-assisted filtration with anodic aluminum oxide membrane for Raman analysis of algal cells
| Autor: | Ting Wei Liao, Po Sheng Chen, Yi Je Juang, Fang Yu Wen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0106 biological sciences Algal cells Materials science 02 engineering and technology 01 natural sciences law.invention chemistry.chemical_compound symbols.namesake law 010608 biotechnology natural sciences Filtration Polydimethylsiloxane Anodic Aluminum Oxide fungi technology industry and agriculture biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology Filter (aquarium) Membrane chemistry Chemical engineering symbols 0210 nano-technology Droplet evaporation Raman spectroscopy Agronomy and Crop Science |
| Zdroj: | Algal Research. 33:412-418 |
| ISSN: | 2211-9264 |
| DOI: | 10.1016/j.algal.2018.06.022 |
| Popis: | In this study, rapid and direct Raman analysis of microalgae was demonstrated through measurement of algal cells (or algal paste) prepared by microwell-assisted filtration using an anodic aluminum oxide (AAO) membrane. To obtain the algal paste, an array of polydimethylsiloxane (PDMS) microwells with through holes was placed on top of the AAO membrane, after which a vacuum was initiated and a droplet of algal solution dispensed inside the PDMS microwells for a 5-min filtration process. The results showed that the AAO membrane has the lowest background noise compared to other types of filter papers or membranes. Compared to the droplet evaporation method, the “coffee-ring” effect was effectively suppressed when applying our proposed filtration method. The algal cells became stacked inside the microwells, which led to relatively uniform and strong Raman signals across individual algal paste and consistency among different algal pastes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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