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Marciano MA, Ordinola-Zapata R, Cunha TVRN, Duarte MAH, Cavenago BC, Garcia RB, Bramante CM, Bernardineli N, Moraes IG. Analysis of four gutta-percha techniques used to fill mesial root canals of mandibular molars. International Endodontic Journal. Abstract Aim To compare the percentage of gutta-percha, sealer and voids and the influence of isthmuses in mesial root canals of mandibular molars filled with different techniques. Methodology Canals in 60 mesial roots of mandibular first molars were prepared with ProTaper instruments to size F2 (size 25, 0.08 taper) and filled using a single-cone, lateral compaction, System B or Thermafil techniques. An epoxy resin sealer was labelled with Rhodamine-B dye to allow analysis under a confocal microscope. The percentage of gutta-percha, sealer and area of voids was calculated at 2, 4 and 6 mm from the apex, using Image Tool 3.0 software. Statistical analysis was performed using nonparametric Kruskal–Wallis and Dunn tests (P |