Differences in the Electrophysiological Properties of Mouse Somatosensory Layer 2/3 NeuronsIn Vivoand Slice Stem from Intrinsic Sources Rather than a Network-Generated High Conductance State
| Autor: | John A. White, Fernando R. Fernandez, Bahar Rahsepar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Membrane potential
0303 health sciences Chemistry General Neuroscience food and beverages Conductance General Medicine Somatosensory system High-conductance state 03 medical and health sciences Electrophysiology chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure In vivo medicine Tetrodotoxin Biophysics Pyramidal cell 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | eneuro. 5:ENEURO.0447-17.2018 |
| ISSN: | 2373-2822 |
| DOI: | 10.1523/eneuro.0447-17.2018 |
| Popis: | Synaptic activityin vivocan potentially alter the integration properties of neurons. Using recordings in awake mice, we targeted somatosensory layer 2/3 pyramidal neurons and compared neuronal properties with those from slices. Pyramidal cellsin vivohad lower resistance and gain values, as well as broader spikes and increased spike frequency adaptation compared to the same cells in slices. Increasing conductance in neurons using dynamic clamp to levels observedin vivo, however, did not lessen the differences betweenin vivoand slice conditions. Further, local application of tetrodotoxin (TTX)in vivoblocked synaptic-mediated membrane voltage fluctuations but had little impact on pyramidal cell membrane input resistance and time constant values. Differences in electrophysiological properties of layer 2/3 neurons in mouse somatosensory cortex, therefore, stem from intrinsic sources separate from synaptic-mediated membrane voltage fluctuations. |
| Databáze: | OpenAIRE |
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