On-demand electrophoretic separation of DNA in written gel lines on planar substrates

Autor:	Peter Koltay, L. Riegger, Laurent Tanguy, Roland Zengerle, Ludwig Gutzweiler
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Materials science technology industry and agriculture Analytical chemistry chemistry.chemical_element Polymer Electrophoresis chemistry.chemical_compound Planar Capillary electrophoresis chemistry On demand Molecule Platinum DNA
Zdroj:	2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII).
DOI:	10.1109/transducers.2013.6626995
Popis:	We report a new approach to perform on-demand electrophoretic separation of DNA. In contrast to standard chip-based capillary electrophoresis in micromachined glass chips, we apply a planar polyimide substrate, write 200 μm wide gel lines bridging two Pt-electrodes and inject 500 pl sample volumes in non-contact manner. The gel is covered with mineral oil to inhibit evaporation. Subsequently, an electrical field is applied for 80 s and the separation of the DNA molecules (56 bp-Cy5 and 112 bp-Cy5, 10 μM) is successfully demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::89a1c8ebb4bb8f46df5b09efc274efed https://doi.org/10.1109/transducers.2013.6626995 Zobrazit plný text záznamu