Effect of hydrogen peroxide on the dehydrogenase and quinone-reductase activity of irradiated Lactobacillus plantarum cells
| Autor: | Turs Selga, Olga Muter, Toms Kusins, Mara Grube, Gunta Kizane, Inga Balode, Andrejs Grinbergs, Vasilijs Bankovskis, Liva Mazkalnina, Kristine Kalneniece, Karlis Shvirksts |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Antioxidant food.ingredient medicine.medical_treatment Dehydrogenase 01 natural sciences Tryptic soy broth chemistry.chemical_compound 0404 agricultural biotechnology food Quinone Reductases 010608 biotechnology medicine Agar Food science Hydrogen peroxide chemistry.chemical_classification biology food and beverages 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Enzyme chemistry Lactobacillus plantarum Food Science |
| Zdroj: | LWT. 134:110236 |
| ISSN: | 0023-6438 |
| Popis: | The resistance of lactobacilli to oxidative stress is of great importance for their applicability as probiotics. This study aimed to evaluate the response of Lactobacillus plantarum strain ATCC® 14917™, grown in either de Man, Rogosa, and Sharpe agar (MRS medium) or tryptic soy broth (TSB medium), to 1–2 mM H2O2 after the exposure to different doses of ionising radiation. Two bacterial extracellular enzyme groups, dehydrogenases (DHAs) and quinone reductases (QRs), served as the criteria of viability and antioxidant activity, respectively. The irradiated L. plantarum culture grown in TSB showed increased QR activity at irradiation doses of 2–50 Gy, with the maximum activity at 10 Gy, compared to non-irradiated cells. The QR activity of the MRS-grown culture was increased significantly (p |
| Databáze: | OpenAIRE |
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