The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature
| Autor: | Yong Qian, Weifeng Liu, Hongming Lou, Yuxia Pang, Xueqing Qiu, Cheng Cai, Feiyun Li, Feiyang Qin |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Green Chemistry. 23:2738-2746 |
| ISSN: | 1463-9270 1463-9262 |
| Popis: | In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (Mw = 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions via cooling. Compared with octadecyl sulfobetaine (SB3–18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3–18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol. |
| Databáze: | OpenAIRE |
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