Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes
| Autor: | Nan-nan Xiao, Ying-lin Deng, Peilong Sun, Pengfei He, An-qiang Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Chromatography
Polymers and Plastics biology Chemistry Organic Chemistry Mannose biology.organism_classification High-performance liquid chromatography Fucose chemistry.chemical_compound Galactose Materials Chemistry Water soluble polysaccharides Sugar Two-dimensional nuclear magnetic resonance spectroscopy Flammulina |
| Zdroj: | Carbohydrate Polymers. 87:2279-2283 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2011.10.061 |
| Popis: | A novel water-soluble heteropolysaccharide FVP60-B was extracted from the fruiting bodies of Flammulina velutipes with boiling water and purified by Sephacryl S-300 and S-400, which molecular weight was estimated to be 1.3 × 10 4 Da by HPLC. It is composed of l -fucose, d -mannose, d -glucose and d -galactose in a ratio of 1.16:0.82:1.00:3.08. Sugar analysis, methylation analysis together with 1 H, 13 C and 2D NMR spectroscopy disclosed that FVP60-B is consisted of a α-(1 → 6)- d -galactopyranan backbone with a terminal fucosyl, terminal glucosyl and α-(1 → 6)- d -mannopyranan units on O -2 of 2,6- O -substituted- d -galactosyl units. |
| Databáze: | OpenAIRE |
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