Tube formation in the first trimester placental trophoblast cells: Differential effects of angiogenic growth factors and fatty acids
| Autor: | Sanjay Basak, Srinivas Vilasagaram, Mrinal K. Das, Abhilash D. Pandya, Asim K. Duttaroy, Arnab Sarkar |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Tube formation medicine.medical_specialty Cell growth Chemistry Trophoblast Cell Biology General Medicine In vitro Fatty acid-binding protein 03 medical and health sciences Vascular endothelial growth factor A 030104 developmental biology 0302 clinical medicine Endocrinology medicine.anatomical_structure Docosahexaenoic acid 030220 oncology & carcinogenesis Internal medicine VEGF Signaling Pathway medicine |
| Zdroj: | Cell Biology International. 40:652-661 |
| ISSN: | 1065-6995 |
| Popis: | The study aims to investigate whether cytosolic fatty acid-binding protein-4 (FABP4) is involved in angiogenic growth factors- and fatty acid-induced tube formation in first trimester placental trophoblast cells, HTR8/SVneo. We determined the tube formation both at basal as well as stimulated levels in the absence and presence of inhibitors of FABP4 and VEGF signaling pathways. Basal level of tube formation was maximally reduced in the presence of 50 µM of FABP4 inhibitor compared with those by VEGF signaling pathway inhibitors (rapamycin, L-NAME, and p38 MAP kinase inhibitor). Whereas docosahexaenoic acid, 22:6n-3 (DHA)-, and VEGF-induced tube formation was maximally inhibited by p38 MAP kinase inhibitor (63.7 and 34.5%, respectively), however, leptin-induced tube formation was inhibited maximally by FABP4 inhibitor (50.7%). ANGPTL4 and oleic acid (OA)-induced tube formation was not blocked by any of these inhibitors. The FABP4 inhibitor inhibited cell growth stimulated by DHA, leptin, VEGF, and OA (P |
| Databáze: | OpenAIRE |
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