Callus Derived Regeneration of Some Selected Brassica Genotypes
| Autor: | M T Rahman, S Ahmmed, S K Biswas, M. Z. Tareq, A B M Z Hoque |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | The Agriculturists. 15:1-10 |
| ISSN: | 2304-7321 1729-5211 |
| Popis: | The experiment was conducted to observe the callus induction ability of Brassica species. Plantlets were regenerated from cotyledon and stem explants of Brassica napus , Brassica campestris and Brassica juncea through direct organogenesis. The experiments were conducted in a Completely Randomized Design (CRD) with 4 replications. The highest frequency of callus formation was recorded in MS containing 2.0 mgl -1 BAP, 0.5 mgl -1 NAA and 2.0 mgl -1 AgNO 3 in both stem and cotyledon explants. Among these explants, stem was found to be better responsive in callus induction than cotyledon. Among the genotypes used, BINA Sarisha-4 induced the highest percentage (100.00%) of callus from stem explants which was followed by BINA Sarisha-5 (100.00%) and Sampad (83.35%). On the other hand, BINA Sarisha-4 induced callus from 91.67% cotyledon explants, followed by BINA Sarisha-5 (75.00%) and Sampad (66.67%). Similarly, the highest percentage of shoot regeneration (58.34%) from stem explants of BINA Sarisha-4 was observed in MS medium supplemented with combination of hormone and silver nitrate concentrations. The highest percentage of root induction was 66.67 and 58.33% in plantlets derived from stem and cotyledon explants, respectively in ½ MS medium supplemented with 2.0 mgl -1 IBA and 0.5 mgl -1 of NAA. The highest survival rate was found after acclimatization of plants derived from stem (77.78%) and cotyledon (64.28%) explants of BINA Sarisha-4 in pot and 64.33 and 55.55%, respectively in field. The Agriculturists 2017; 15(2) 01-10 |
| Databáze: | OpenAIRE |
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