Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures
| Autor: | Priscila de Queiroz Souza Passos, Leticia Bonfim, Karina de Oliveira Gonçalves, Daniel Perez Vieira, Flávia Rodrigues de Oliveira Silva, Lilia Coronato Courrol |
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| Rok vydání: | 2019 |
| Předmět: |
Materials Science (miscellaneous)
Nanochemistry Nanoparticle 02 engineering and technology Cell Biology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Atomic and Molecular Physics and Optics 0104 chemical sciences chemistry.chemical_compound Magnetization 3D cell culture Membrane chemistry Chemical engineering Cell culture Electrical and Electronic Engineering Physical and Theoretical Chemistry 0210 nano-technology Iron oxide nanoparticles Magnetic levitation Biotechnology |
| Zdroj: | Applied Nanoscience. 9:1707-1717 |
| ISSN: | 2190-5517 2190-5509 |
| DOI: | 10.1007/s13204-019-00962-1 |
| Popis: | The use of three-dimensional cell cultures has been widely used for efficacy and/or toxicity testing of compounds. One of the most promising systems, based on magnetic levitation, is dependent on proper cell magnetization, achieved through adsorption of iron-oxide nanoparticles on cell membranes. These particles must bare not only significant responses to magnetic fields, but also a stable mechanism to attachment to cells. This work proposes a simple, one-pot synthesis method to produce magnetite nanoparticles, using a Fe2+ precursor associated with amino acids under microwave heating, and successive steps to confer positive charges to particles. X-ray diffraction could confirm Fe3O4 composition, and TEM analysis showed cubic-like crystallites with less than 50 nm. Zeta-potential experiments showed that particles remained positively charged (20.98 ± 0.28 mV) in physiological pH, suggesting ability to attach to (negatively charged) cell membranes, observed through optical microscopy. Iron colloid was found to be non-cytotoxic in concentrations up to 8% in cell culture media. Finally, human prostate cancer cells were cultured in 96-well plates using magnetic levitation and could be kept 8 days in culture. The results showed a feasible way to produce spheroids relying on magnetic levitation, using a newly described method of magnetic and cell adherent nanoparticle production. |
| Databáze: | OpenAIRE |
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