The separation of date (Phoenix dactylifera) sterols by liquid chromatography

Autor: Nobu Kikuchi, Taihei Miki
Rok vydání: 1978
Předmět:
Zdroj: Mikrochimica Acta. 69:89-96
ISSN: 1436-5073
0026-3672
DOI: 10.1007/bf01196983
Popis: Isolation of a crystalline plant sterol mixture from the sarcocarp of the date was followed by successive extraction and column chromatography. The composition of the mixture was examined by gas chromatography. An additional gas chromatographic-mass spectroscopic identification of the sterol mixture was carried out after conversion of the sample into its trimethylsilyl derivates. Cholesterol, campesterol, stigmasterol,β-sitosterol and isofucosterol were found from the identification of molecular ions, the assignment of fragment ions and the comparison of each spectral feature with that of the authentic compound. The sterol mixture was then examined by high-performance liquid chromatography employing a “Zorbax ODS” column and an acetonitrile-water-0.1M sodium acetate (90∶10∶1) solvent system, and the peaks were detected with radiation at 210 nm. Six peaks were found on the chromatogram. The identification of each peak by means of gas chromatography and infrared spectroscopy was followed by the collection of each component. The peaks were an unknown (ghost) peak, isofucosterol, cholesterol, stigmasterol + campesterol, campesterol andβ-sitosterol, successively. The plant sterol mixture has only been analysed on reverse-phase column packings whereas the hormonal corticosteroids and male or female hormonal steroids have been separated on both normal and reverse-phase column packings.
Databáze: OpenAIRE
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