Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (Lupinus albus L.) Represent a Unique Genepool

Autor:	Raymond Cowley, Rosy Raman, David Luckett, Harsh Raman
Rok vydání:	2014
Předmět:	Germplasm Genetic diversity Dart biology Diversity Arrays Technology UPGMA food and beverages biology.organism_classification White (mutation) Lupinus Agronomy Botany Cultivar human activities computer computer.programming_language
Zdroj:	Open Journal of Genetics. :87-98
ISSN:	2162-4461 2162-4453
DOI:	10.4236/ojgen.2014.42012
Popis:	PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::89592d669a33dc923a2f5b7427a2b50c https://doi.org/10.4236/ojgen.2014.42012 Zobrazit plný text záznamu