Su1962 Induction of Aldehyde Dehydrogenase-2 (Aldh2) Expression in Esophageal Epithelial Cells Suppresses the Acetaldehyde-Mediated DNA Damage
| Autor: | Mihoko Tsurumaki, Shin'ichi Miyamoto, Shinya Ohashi, Yukie Nakai, Tomonari Matsuda, Tsutomu Chiba, Yusuke Amanuma, Hiroshi Nakagawa, Manabu Muto |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Hepatology biology business.industry Stomach Gastroenterology Aldehyde dehydrogenase Histology Inflammation medicine.disease_cause biology.organism_classification Malondialdehyde Aloe vera Proinflammatory cytokine chemistry.chemical_compound medicine.anatomical_structure chemistry Internal medicine biology.protein Medicine medicine.symptom business Oxidative stress |
| Zdroj: | Gastroenterology. 146:S-509 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/s0016-5085(14)61841-2 |
| Popis: | AIM: Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the most commonly prescribed worldwide and known to induce gastric injury from multiple mechanisms. Aloe vera is known to effectively decrease inflammation and promote ulcer healing but there are still limited data. Therefore, this study performs to evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin-induced gastropathy. METHODS: Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control group, n = 6) were given orally distilled water (DW) at 0 and 4 hours. Group 2 [indomethacin (IMN) group, n = 6] were given orally IMN (150 mg/kg) dissolved in 5% NaHCO3at time 0 and 4 hours. Group 3 (Aloe vera-treated group, n = 6) were given orally Aloe vera (150 mg/kg) dissolved in DW and IMN at 0 and 4 hours. At 8 hours after the first dose, the rat's stomach was removed to determine gastric malondialdehyde (MDA), the number of interleukin-18 (IL-18) positive stained cells (%) by immunohistochemistry method, and histopathological examination. Serum was collected to determine the level of tumor necrosis factorα (TNFα) and Cytokine-induced neutrophil chemoattractant-1 (CINC-1) (by ELISA method). RESULTS: In the IMN group, serum TNF-α, CINC-1and gastric MDA significantly increased when compared to the control group (27.8±1.5 vs. 85.1±49.1 pg/mL, 104.5 ± 45.8 vs. 1054.7 ± 20.4 pg/mL and 1.7±0.2 vs. 9.4±1.1 nmol/mg protein, p |
| Databáze: | OpenAIRE |
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