Features of connective tissue structure of ligaments of the uterus in female genital prolapse
| Autor: | T A Demura, M L Khanzadyan |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Kazan medical journal. 96:498-505 |
| ISSN: | 2587-9359 0368-4814 |
| DOI: | 10.17750/kmj2015-498 |
| Popis: | Aim. To study the features of collagen I and III deposition and elastin in uterosacral ligaments and round ligaments of uterus in females with genital prolapse. Methods. The study was performed on surgical specimens and biopsies of uterosacral ligaments and round ligaments of uterus of 15 females with genital prolapse and 15 females without prolapse who also underwent metrectomy using abdominal access. Four tissue specimens were obtained from every patient, biopsy material was fixed in 10% neutral formalin and were embedded in paraffin, and cut for the paraffin slices of 4 μm. Specimens were stained by haematoxylin and eosine, Van Gieson’s picro-fuchsin stain and Weigert’s Resorcin-Fuchsin stain, immunohistochemistry (immunoperoxidase reactions) with primary antibodies to collagen I and III and elastin were also performed. Results. Morphologic study of the ligaments of the uterus in females with genital prolapse revealed fibrosis and fragmentation of elastin, diffuse atrophy, hyaline or mucinous degeneration of smooth muscle and extracellular matrix edema. Connective tissue disarrangement at genital prolapse was seen as decreased expression of collagen type I and elastin. Decreased elasticity determined over-stretching of uterosacral ligaments and round ligaments of uterus, which decreased microcirculation and promoted the ischemia of ligaments of uterus connective tissue. Disproportion of connective tissue proteins expression was enhanced by imbalance of collagen I and III, with the last, which is the least strong, prevailing. Stereotypic changes in the ligaments of uterus structures, seen as misallocation and placing of collagen and elastin fibers and, consequently, loosening of the elastic and collagen fibers, were revealed in patients with genital prolapse. Conclusion. Revealed features of collagen I and III and elastin deposition in uterosacral ligaments and round ligaments of uterus in females of reproductive age with genital prolapse allow predicting the risk for development and relapses of genital prolapse. |
| Databáze: | OpenAIRE |
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