Effect of the conditions of isolation on the physicochemical properties of human serum albumin in the norm and with pathology

Autor: V. V. Nikolaichik, R. G. Zhbankov, V. V. Sarnatskaya, A. I. Ivanov, L A Yushko, L. A. Meleshchenko, E. V. Korolik, V. G. Nikolaev, E. A. Korolenko
Rok vydání: 1997
Předmět:
Zdroj: Journal of Applied Spectroscopy. 64:29-35
ISSN: 1573-8647
0021-9037
Popis: Differential scanning calorimetry and IR spectrosocopy were used to investigate the effect of the procedure of isolation of human serum albumin on its physicochemical characteristics. It is shown that fractionation of blood plasma with ethylene glycol followed by ion exchange chromatography can be used to obtain albumin of normal donors that is similar to the albumin in the nonfractionated plasma according to melting thermograms. Endotherms of human serum albumin samples that were obtained by affinity chromatography and preparative electrophoresis are bimodal, unlike the monophasic for albumin obtained by polyethylene glycol precipitation. These changes result from a higher content of nonetherified fatty acids in the albumin samples obtained by affinity chromatography and from modification of the secondary protein structure in the samples obtained by electrophoresis. Analysis of melting thermograms of serum albumin from patients with uremia, chronic hepatitis, and peritonitis shows that fractionation of blood with polyethylene glycol preserves the thermodynamic characteristics of the various pathological serum albumins to the greatest extent. The present results demonstrate the advantage of polyethylene glycol fractionation for isolation of native preparations of normal and “pathological” human serum albumin.
Databáze: OpenAIRE
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