| Autor: |
Ingo Nindl, Eggert Stockfleth, J. Roewert, Marc Gottschling, Chantip Dang, T. Forschner |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
British Journal of Dermatology. 155:763-770 |
| ISSN: |
0007-0963 |
| DOI: |
10.1111/j.1365-2133.2006.07401.x |
| Popis: |
Summary Background Tenascin-C (Tn-C) is an extracellular matrix protein with multiple functions that is present at low levels in normal tissues, but which is highly present in various tumours. The mRNA expression and protein level of Tn-C including its various isoforms have not been investigated comprehensively so far in cutaneous squamous cell carcinoma (SCC) and the precursor lesion actinic keratosis (AK). Objectives To assess the dysregulated expression and splice variants of Tn-C in cutaneous squamous cell dysplasia and carcinoma. Methods Biopsies from 66 patients (or representative subsets) that comprised 25 specimens from normal skin, 19 AK and 22 cutaneous SCC were analysed for Tn-C splice variants using splice-specific primers. The amount of Tn-C mRNA was investigated by quantitative real-time reverse transcription–polymerase chain reaction. In addition, the presence of Tn-C protein was analysed in sections of paraffin-embedded tissues using immunohistochemistry. Results The large Tn-C splice variant was present in only 5% of normal skin samples, in comparison with 63% of AK (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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