Electron−Phonon Coupling in Solubilized LHC II Complexes of Green Plants Investigated by Line-Narrowing and Temperature-Dependent Fluorescence Spectroscopy
| Autor: | R. Schödel, and J. Voigt, J. Pieper, G. Renger, ‖ K.-D. Irrgang |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | The Journal of Physical Chemistry B. 105:7115-7124 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp010229g |
| Popis: | Line-narrowed and temperature-dependent fluorescence spectra are reported for the solubilized trimeric light-harvesting complex of Photosystem II (LHC II). Special attention has been paid to eliminate effects owing to reabsorption and to ensure that the line-narrowed fluorescence spectra are virtually unaffected by hole burning or scattering artifacts. Analysis of line-narrowed fluorescence spectra at 4.2 K indicates that the lowest Qy-state of LHC II is characterized by weak electron−phonon coupling with a Huang−Rhys factor of ∼ 0.9 and a broad and strongly asymmetric one-phonon profile with a peak frequency ωm of 15 cm-1 and a width of Γ = 105 cm-1. The 4.2 K fluorescence data are further consistent with the assignment of the lowest Qy-state at ∼ 680.0 nm and an inhomogeneous width of ∼80 cm-1 gathered from a recent hole-burning study (Pieper et al. J. Phys. Chem. A 1999, 103, 2412). The temperature dependence of the fluorescence spectra of LHC II is simulated using the low-energy Qy-level structure rep... |
| Databáze: | OpenAIRE |
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