Studies on method of collagen-induced platelet aggregation
| Autor: | Hirofumi Watanabe, Junichi Isobe, Nobue Maruden, Masako Kusumoto, Yoko Hamai, Tsunesada Taharaguchi |
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| Rok vydání: | 1979 |
| Předmět: | |
| Zdroj: | Blood & Vessel. 10:267-270 |
| ISSN: | 1884-2372 0386-9717 |
| DOI: | 10.2491/jjsth1970.10.267 |
| Popis: | Preparation procedures of collagen suspension in testing platelet aggregation by Born's method are presented a great deal, but there are few reports on the concentrations of collagen in detail.Recently, we are able to obtain easily a commercial reagent of collagen suspension (Collagenreagent HormR, Hormon-Chemie Munchen, ETC), and then would like to try to be standardized in its method. ETC contains 1mg per ml of native collagen fibrils from equine tendons suspended in an organic isotonic buffer of pH 2.7. Collagen suspension from bovine achilles tendons (Sigma, BTC) treated with 0.03N HCl was prepared as control, which contains the same concentration of collagen as ETC. ETC was used straight from the vial, not diluted with buffer as diluting medium.1) Each concentration of BTC and ETC requires 20μg, 10μg per ml in platelet rich plasma (PRP) at final concentration respectively, which show about fifty percent of aggregation values after five minutes adding each reagent.2) Normal ranges (mean±S. D.) of BTC —and ETC—induced aggregation were 48.6±15.6%, 52.0±12.5% each other.3) We obtained the good reproducibility of these reagents, but there was no difference between BTC and ETC in reliability. The lag times (mean±S. D.) in aggregating process were 47.8±10.2 seconds in BTC and 16.3±1.3 in ETC.4) ETC was fairly stable against heating or freezing than BTC, and no change in aggregating activity of ETC in storage at 4°C was recognized for at least a half year. In this period, we did not see any coarse fibrils of collagen.5) Examing the sensitivity of reagents to collagenase, aggregating activity of ETC was more decreased than that of BTC, and then it was suggested that ETC might be microfibrillar collagen.6) The effects of sucrose on polymerization of monomer collagen were tested and activities in these reagents were reduced with corresponding the dose of sucrose, but no difference was detectable between them.7) We investigated the sensitivities of these reagents to platelet aggregation in 13 samples from patients with aspirin or other antithrombotic drugs ingested and one patient from Glanzmann's thrombasthenia, but there was no difference in aggregation values between these reagents.These data suggest that ETC is available to standardize the test af collagen—induced platelet aggregation. |
| Databáze: | OpenAIRE |
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