Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Medium (hMSC-CM) Improves Antioxidant Status in Carbon Tetrachloride-Induced Oxidative Damage in Rat
| Autor: | Mahdi Bahmani, Mohammad Hashemnia, Aref Salehzadeh, Nasrin Ziamajidi, Roghayeh Abbasalipourkabir |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant General Mathematics medicine.medical_treatment General Physics and Astronomy medicine.disease_cause digestive system Superoxide dismutase Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine chemistry.chemical_classification Liver injury biology Glutathione peroxidase General Chemistry equipment and supplies Malondialdehyde medicine.disease 030104 developmental biology chemistry Catalase 030220 oncology & carcinogenesis Carbon tetrachloride biology.protein General Earth and Planetary Sciences General Agricultural and Biological Sciences Oxidative stress |
| Zdroj: | Iranian Journal of Science and Technology, Transactions A: Science. 44:1327-1335 |
| ISSN: | 2364-1819 1028-6276 |
| DOI: | 10.1007/s40995-020-00944-x |
| Popis: | Carbon tetrachloride (CCl4) as environmental toxin induces tissue damage through oxidative stress. In the present study, the effect of treatment with Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Medium (hMSC-CM) was evaluated on improvement of chronic injectable CCl4-induced oxidative stress. Fifty-four male rats were divided into nine groups: N (PBS-treated group), O8 and O12 (Olive oil-treated group for 8 and 12 weeks, respectively), D8 and D12 (hMSC-CM-treated group for 4 and 8 weeks, respectively), C8 and C12 (CCl4-treated group for 8 and 12 weeks, respectively), T8 (CCl4- and hMSC-treated group resembled C8 and D8, respectively), T12 (CCl4- and hMSC-treated group resembled C12 and D12, respectively). Then, oxidative stress markers were evaluated in the liver tissue. To evaluate histopathological lesions, Masson’s trichrome and H&E stainings were performed. Although treatment with hMSC-CM resulted in a significant increase in the total antioxidant capacity (TAC), total thiol groups (TTG), and a significant decrease in the total oxidant status (TOS) and MDA (malondialdehyde) compared to CCL4 group, only MDA activity returned to normal base which observed in the control group. Treatment with hMSC-CM increased superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity compared to groups received only CCl4. Also treatment with hMSC-CM improved CCl4-induced histopathological lesions. Results of the present study demonstrated that the hMSC-CM as a potential antioxidant could be an effective therapeutic agent against CCl4-induced liver injury. |
| Databáze: | OpenAIRE |
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