Characterization of the major protein fraction carmin from safflower seeds (Carthamus tinctorius L.) by dynamic light scattering and circular dichroism spectroscopy

Autor:	B. Raab, V. Prakash, D. Zirwer, K. D. Schwenke, K. Gast
Rok vydání:	1989
Předmět:	Circular dichroism Molecular mass biology Chemistry Diffusion Carthamus Analytical chemistry biology.organism_classification Spectral line Crystallography Dynamic light scattering Protein secondary structure Food Science Stokes radius
Zdroj:	Food / Nahrung. 33:929-933
ISSN:	1521-3803 0027-769X
DOI:	10.1002/food.19890331004
Popis:	Hydrodynamic parameters of the major protein fraction carmin from safflower seed were determined by means of dynamic light scattering. The results indicated a translational diffusion coefficient of (3.81 f 0.06) x cm2 s-l and a STOKES radius of (5.6 f 0.06) nm. The calculated molecular mass M,, was (3.1 f 0.1) x lo5 g/mol and the frictional ratio was 1.25 & 0.05. Evaluation of secondary structure from CD spectra using the method of PROVENCHEanRd GL~CKNgEaRve 8 % a-helix, 45 % 8-sheet and 47 % aperiodic structure. These results are discussed in view of structural homology of oilseed proteins.
Databáze:	OpenAIRE
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