| Autor: |
Maria Walle, Sebastian Wrighton, Johannes Kumra Ahnlide, Johan Malmström, Magdalena Godzwon, Lars Malmström, Urban Höglund, Vidar Wendel-Hansen, Olivia Larsson, Martin Sundwall, Pontus Nordenfelt, Magnus Rasmussen, Elizabeth Elder, Wael Bahnan, Anna Bläckberg, Mats Ohlin, Robert Pa Wallin, Hamed Khakzad, Thomas Hellmark, Lotta Happonen, Oscar André |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Spike-specific antibodies are central to effective COVID19 immunity. Research efforts have focused on antibodies that neutralize the ACE2-Spike interaction but not on non-neutralizing antibodies. Antibody-dependent phagocytosis is an immune mechanism enhanced by opsonization, where typically, more bound antibodies trigger a stronger phagocyte response. Here, we show that Spike-specific antibodies, dependent on concentration, can either enhance or reduce Spike-bead phagocytosis by monocytes independently of the antibody neutralization potential. Surprisingly, we find that both convalescent patient plasma and patient-derived monoclonal antibodies lead to maximum opsonization already at low levels of bound antibodies and is reduced as antibody binding to Spike protein increases. Moreover, we show that this Spike-dependent modulation of opsonization seems to affect the outcome in an experimental SARS-CoV-2 infection model. These results suggest that the levels of anti-Spike antibodies could influence monocyte-mediated immune functions and propose that non-neutralizing antibodies could confer protection to SARS-CoV-2 infection by mediating phago-cytosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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