PMO-030 Soluble plant fibres, particularly leek and plantain, inhibit adherence of diarrhoea-associated pathogensC difficileand enterotoxigenicEscherichia colito intestinal epithelial cells
| Autor: | Carol L. Roberts, Jonathan M. Rhodes, Barry J. Campbell, Hannah L. Simpson |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Gut. 61:A85.2-A85 |
| ISSN: | 1468-3288 0017-5749 |
| Popis: | Introduction It has long been thought that dietary fibre promotes intestinal health and we have previously shown that the soluble non-starch polysaccharide (NSP) from plantain bananas ( Musa spp.) can inhibit the epithelial adhesion and microfold (M)-cell translocation of Crohn9s-associated Escherichia coli ( Gut 2010; 59 :1331–9) and a range of diarrhoeal pathogens including Salmonella and Shigella ( Gut 2011;60:A96). Here we examined NSP from representative examples of monocotyledon and dicotyledon plant fibres, for efficacy to inhibit epithelial interactions of Clostridium difficile , a major cause of antibiotic associated diarrhoea, and enterotoxigenic E coli (ETEC), the commonest cause of traveller9s diarrhoea. Methods Human intestinal epithelial cell-line Caco2-cl1 cell monolayers were pre-treated for 30 min with NSP preparations, including those from vegetable (broccoli, leek), fruit (plantain, apple) and cereal (oat) sources, followed by infection with C. difficile (for 2 h, at multiplicity of infection MOI of 100) or ETEC (4 h; MOI 100). In parallel experiments, adherence of bacteria to cell monolayers was examined following fixation, Giemsa stain and light microscopy. Results C difficile adhesion to Caco2-cl1 cells was significantly inhibited in the presence of broccoli, leek and plantain NSP. Leek NSP, at 5 mg/ml, had the most significant effect on inhibition of C difficile adhesion (54.9±9.7% reduction) compared to untreated controls (n=3−9, p C difficile adhesion to CaCo2-cl1 cells. ETEC adhesion to CaCo2-cl1 cells was also significantly inhibited in the presence of leek NSP (53.7±13.6%; p Conclusion Leek, plantain and/or broccoli NSP show efficacy at blocking C difficile and ETEC adhesion in a dose dependent manner to the intestinal epithelium in vitro and at concentrations readily achievable in vivo. The close proximity of C difficile and ETEC to the host epithelium is almost certainly essential for the release of their respective toxins and the exertion of their pathogenic effect. Disruption of bacterial-epithelial adherence to the intestinal mucosa by soluble plant fibres may therefore be of therapeutic benefit. Competing interests H Simpson grant/research support from: industrial case studentship with support from Provexis plc, C Roberts conflict with: past employee of Provexis plc, J Rhodes consultant for: is a member of advisory boards for Atlantic, Procter and Gamble and Falk, speaker bureau with: Has received speaking honoraria from Abbott, Falk, Ferring, Glaxo Smith Kline, Procter and Gamble, Schering Plough, Shire and Wyeth, Conflict with: holds a patent with the University of Liverpool and Provexis UK for use of a soluble fibre preparation as maintenance therapy for Crohn9s disease plus a patent pending for its use in antibiotic-associated diarrhoea, B Campbell grant/research support from: grant support from Provexis plc and the Bo & Vera Ax:son Johnson Foundation Ltd. |
| Databáze: | OpenAIRE |
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