Imaging Nanoscale Heterogeneity in Ultrathin Biomimetic and Biological Crystals
| Autor: | Tengyue Jian, Chun-Long Chen, A. Scott Lea, James E. Evans, Kevin T. Crampton, Patrick Z. El-Khoury, Brian T. O'Callahan, Irina V. Novikova, Markus B. Raschke |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Infrared Ensemble averaging Peptoid 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Crystal chemistry.chemical_compound General Energy Membrane chemistry Optical microscope Chemical physics law Physical and Theoretical Chemistry 0210 nano-technology Spectroscopy Nanoscopic scale |
| Zdroj: | The Journal of Physical Chemistry C. 122:24891-24895 |
| ISSN: | 1932-7455 1932-7447 |
| Popis: | The mechanisms of interface biochemistry are often obscured through ensemble averaging of complex networks of proteins that exhibit varying degrees of structural heterogeneity. Here, we perform nanometer spatially resolved chemical spectroscopy using infrared vibrational scattering-scanning near-field optical microscopy (IR s-SNOM) to image protein–protein interactions and conformational heterogeneity of (i) a two-dimensional lipid-like peptoid membrane and (ii) a three-dimensional catalase crystal. In the peptoid membrane, which consists of stacked ∼4 nm high peptoid layers, spatio-spectral line width analyses reveal heterogeneity of the vibrational dynamics due to peptoid–substrate and peptoid–peptoid interactions. In s-SNOM imaging of catalase crystals, our results revealed complex secondary structures that vary over nanometer length scales. Broadening of vibrational resonances, as observed from the dehydrated catalase crystals, indicates a higher degree of heterogeneity as compared to the synthetic pe... |
| Databáze: | OpenAIRE |
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