Effects of Bacillus subtilis on the water quality, stress tolerance, digestive enzymes, growth performance, immune gene expression, and disease resistance of white shrimp (Litopenaeus vannamei) during the early hatchery period

Autor: M Mirbakhsh, Saeid Hosseinzadeh, Masoud Mahjoub, M. Afsharnasab, Mostafa Sayyadi, Shapoor Kakoolaki
Rok vydání: 2021
Předmět:
Zdroj: Aquaculture International. 29:2489-2506
ISSN: 1573-143X
0967-6120
DOI: 10.1007/s10499-021-00758-7
Popis: In the present study, the white shrimp, Litopenaeus vannamei, was supplemented with the probiotic Bacillus subtilis IS02 in order to improve the larval survival rate. Various experimental groups including control (no probiotic in the rearing water), 106, 107, and 108 CFU mL–1 of the probiotic supplemented samples were added to the salt water once every 3 days during the 21 days of breeding. The stress tolerance and immune status were then evaluated. It was shown that the larval development stages and survival rate were significantly improved following application of the 108 CFU mL−1 of the probiotic. Total bacterial and presumptive Vibrio counts were numerically reduced. The stress test revealed a considerable increase in the postlarvae vitality. Moreover, a significant decrease in the cumulative mortality of postlarvae exposed to the probiotic at the level of 108 CFU mL−1 was recorded after a sudden transfer to 300 mg L−1 nitrite-N, compared to that of the control. The analysis of immune-related gene expressions showed the overexpression of the genes prophenoloxidase (proPO) and peroxinectin (PE) in the shrimp fed with water containing 108 CFU mL−1 of probiotic. These findings demonstrate the effects of the administration of B. subtilis IS02 on improving growth and enhancing the immune response, thus increasing resistance to bacterial pathogens.
Databáze: OpenAIRE
Externí odkaz: