| Popis: |
We report on computational and experimental investigations into the effects of high-frequency focused ultrasound stimulation on neuronal tissue. We simultaneously measuring local field potentials as well as extracellular oxygen and potassium concentrations in hippocampal brain slices to investigate physiological responses, including metabolic demand, during ultrasonic stimulation. To better interpret our observations, we utilized computational models that incorporate dynamics for transmembrane ionic and osmotic flows, membrane fluctuations, and metabolism. Our experiments and models suggest that ultrasonic stimulation can produce substantial ionic redistribution that leads to a significant metabolic burden. |
