| Autor: |
Mooney, R.A., Awad, H., Farnsworth, C.W., Chen, D.-G., Brown, M.L., Hilton, M.J., O'Keefe, R.J., Yukata, K., Zuscik, M.J., Xing, L. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| DOI: |
10.17615/j3fx-8v71 |
| Popis: |
Introduction: Impaired healing and non-union of skeletal fractures is a major public health problem, with morbidity exacerbated in patients with diabetes mellitus (DM). DM is prevalent worldwide and affects approximately 25.8 million US adults, with >90% having obesity-related type 2 DM (T2DM). While fracture healing in type 1 DM (T1DM) has been studied using animal models, an investigation into delayed healing in an animal model of T2DM has not yet been performed. Methods: Male C57BL/6J mice at 5 weeks of age were placed on either a control lean diet or an experimental high-fat diet (HFD) for 12 weeks. A mid-diaphyseal open tibia fracture was induced at 17 weeks of age and a spinal needle was used for intra-medullary fixation. Mice were sacrificed at days 7, 10, 14, 21, 28, and 35 for micro-computed tomography (μCT), histology-based histomorphometry and molecular analyses, and biomechanical testing. Results: HFD-fed mice displayed increased body weight and impaired glucose tolerance, both characteristic of T2DM. Compared to control mice, HFD-fed mice with tibia fractures showed significantly (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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