Sensitization of Guinea Pigs to Herpes Simplex Virus
| Autor: | Howell W. Rogers, L. Vernon Scott, Robert A. Patnode |
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| Rok vydání: | 1972 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 109:801-806 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.109.4.801 |
| Popis: | Guinea pigs were sensitized to herpes simplex virus (HSV) by inoculation with infective virus, inactive virus or a HSV soluble antigen (SA) preparation. Sensitivity was assayed by means of skin tests with SA. The largest skin reactions (erythema and induration) were observed in guinea pigs that had received two sensitizing injections of infective HSV. Inactive HSV was found to be an effective sensitizing material, suggesting that production of cutaneous hypersensitivity to HSV can occur in the absence of viral infection. A single intracutaneous injection of SA also sensitized animals to a subsequent skin test with SA. Based upon the timing of maximal response, the mononuclear nature of the cellular infiltrate at 24 hr and non-transferability of reactivity with serum by the local passive transfer technique, the skin reactions observed in SA-sensitized animals are believed to be those of classical delayed hypersensitivity. While the skin reactions seen in these animals were not as large as those observed in animals sensitized with infective or inactivated HSV, they were shown to be specific for SA. Animals sensitized with infective vaccinia virus or L-cell control antigen did not respond to skin tests with SA. |
| Databáze: | OpenAIRE |
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