Effect of levodopa/carbidopa on stress response in zebrafish
| Autor: | Caio Maximino de Oliveira, Aline Pompermaier, Renan Idalencio, Taise Miranda Lopes, Leonardo José Gil Barcellos, Michele Fagundes, Suelen Mendonça Soares, Heloísa Helena de Alcantara Barcellos, Fabiana Kalichak |
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| Rok vydání: | 2021 |
| Předmět: |
Levodopa
medicine.medical_specialty Physiology 030310 physiology 03 medical and health sciences Behavioral Neuroscience AMPT Norepinephrine 0302 clinical medicine Dopamine Internal medicine medicine Zebrafish Ecology Evolution Behavior and Systematics 0303 health sciences biology Tyrosine hydroxylase Chemistry Dopaminergic biology.organism_classification nervous system diseases Endocrinology Carbidopa Animal Science and Zoology 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Comparative Physiology A. 207:393-399 |
| ISSN: | 1432-1351 0340-7594 |
| DOI: | 10.1007/s00359-021-01479-1 |
| Popis: | The dopaminergic system of zebrafish is complex and the numerous pathways and receptors in the central nervous system (CNS) are being extensively studied. A critical factor for the synthesis, activation and release of catecholamines (CAs) is the presence of tyrosine hydroxylase, an enzyme which converts L-tyrosine into levodopa. Levodopa thus is the intermediary in the synthesis of dopamine (DA) and norepinephrine (NE) and promotes its release; therefore, CAs play an important role in the CNS with hormonal functions. Here, we use levodopa/carbidopa to clarify the involvement of the dopaminergic pathway in the stress response in zebrafish submitted to an acute stress challenge. Acute stress was induced by chasing fish with a net for 2 min and assessed by measuring whole-body cortisol levels. Two experiments were carried out, the first with exposure to levodopa/carbidopa and the second with exposure to AMPT and levodopa/carbidopa. Levodopa/carbidopa balances the stress response through its action on the zebrafish hypothalamic–pituitary–adrenal (HPA) axis. Changes in cortisol levels suggest that DA was related to the balance of the stress response and that NE decreased this response. These effects were specific to stress since levodopa/carbidopa did not induce changes in cortisol in non-stressed fish. |
| Databáze: | OpenAIRE |
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