Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer
| Autor: | Mohammadhossein Ahmadi Borhanabadi, Mohammad Amin Raeisi Estabragh, Gholamreza Dehghannoudeh, Ibrahim M Banat, Mandana Ohadi, Mohammad Hassan Moshafi |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Jundishapur Journal of Natural Pharmaceutical Products. |
| ISSN: | 2228-7876 1735-7780 |
| DOI: | 10.5812/jjnpp-134325 |
| Popis: | Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties. Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel. Methods: A. junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead's morphology. Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hardening time. A. junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design. Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants. |
| Databáze: | OpenAIRE |
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