Durchflusszytometrie zum Auffinden verbesserter und neuer Enzyme

Autor:	Uli Schwaneberg, Milan Blanusa, Frank Niehaus
Rok vydání:	2013
Předmět:	chemistry.chemical_classification medicine.diagnostic_test Pharmacology toxicology Computational biology Biology Bioinformatics Human genetics Protein evolution Flow cytometry Enzyme chemistry medicine Molecular Biology Biotechnology
Zdroj:	BIOspektrum. 19:45-48
ISSN:	1868-6249 0947-0867
DOI:	10.1007/s12268-013-0271-7
Popis:	Flow cytometry screening systems in emulsions offer a throughput (up to 108) that would enable novel strategies in directed protein evolution (e. g. with high mutational loads) and enzyme discovery in metagenomes (hit rates < 1 in 105). A main challenge which hampers a routinely use of flow cytometry is the lack of suitable screening assays. This article highlights the potential of flow cytometry to improve a monooxygenase with high mutational loads and to discover novel enzymes in metagenomes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::88c9f6c5191d1993c10a7f0ff76ac611 https://doi.org/10.1007/s12268-013-0271-7 Zobrazit plný text záznamu Full text from SpringerLink