The calcium binding protein EhCaBP6 is a microtubular-end binding protein inEntamoeba histolytica

Autor:	Narendra Padhan, Jaspreet Singh Grewal, Alok Bhattacharya, Saima Aslam, Anuradha Lohia
Rok vydání:	2013
Předmět:	biology Binding protein Immunology Endocytosis biology.organism_classification Microbiology Cell biology Entamoeba histolytica Microtubule Cytoplasm Virology Calcium-binding protein parasitic diseases Cytokinesis Binding domain
Zdroj:	Cellular Microbiology. 15:2020-2033
ISSN:	1462-5814
DOI:	10.1111/cmi.12167
Popis:	Summary The genome of Entamoeba histolytica encodes several calcium binding proteins and those characterized thus far have been shown to participate predominantly in phagocytosis and endocytosis. Our study showed that EhCaBP6 has two EF-hand domains EFI and EFIII; it can bind Ca2+ in vitro and undergoes conformational transition on binding Ca2+ suggesting that it can serve as a calcium signal sensor. EhCaBP6 is localized in the nucleus, cytoplasm and plasma membrane and is sensitive to heat stress. Unlike other Ca2+ binding proteins that have been studied in E. histolytica, EhCaBP6 is found at microtubule ends and at the intercellular bridge with the microtubules during cytokinesis. Furthermore, increased expression of EhCaBP6 was correlated with a significant increase in the number of microtubular structures suggesting that this protein may regulate chromosome segregation and cytokinesis in E. histolytica.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::88966a6c3d988fa02e63e5f6b259134e https://doi.org/10.1111/cmi.12167 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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