In vitro plant regeneration and ethylmethanesulphonate (EMS) uptake in somatic embryos of date palm (Phoenix dactylifera L.)

Autor:	M. S. Omar, F. J. Novák
Rok vydání:	1990
Předmět:	chemistry.chemical_classification Somatic embryogenesis Picloram Mutagen Horticulture Biology medicine.disease_cause Tissue culture chemistry.chemical_compound chemistry Auxin Callus Botany Dicamba medicine Phoenix dactylifera
Zdroj:	Plant Cell Tissue and Organ Culture (PCTOC). 20:185-190
ISSN:	1573-5044 0167-6857
Popis:	The effect of the auxins dicamba (3,6-dichloro-2-methoxybenzoic acid) and picloram (4-amino-3,5,6-trichloropicolinic acid) on callus growth and embryogenesis in Phoenix dactylifera L. was investigated. Maximum callus fresh weight was obtained in nutrient medium enriched with 200 µm picloram. Somatic embryogenesis and subsequent plant regeneration was achieved following transfer of such calli to hormone-free medium. Germination of the somatic embryos was influenced by treatment with the chemical mutagen ethylmethanesulphonate (EMS). Uptake of the labelled mutagen ([14C]EMS) by the somatic embryos increased with increased incubation time. Presence of dimethyl sulphoxide (DMSO) as a carrier agent during mutagenic treatment was necessary for efficient mutagen uptake.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8894db81937115251eb3bd7c0c2e6401 https://doi.org/10.1007/bf00041880 Zobrazit plný text záznamu Full text from SpringerLink