Starch-accumulating Sweet Potato Callus Tissue Devoid ofβ-Amylase but with Two Starch Phosphorylase Isozymes
| Autor: | Rong-Huary Juang, Heng-Long Wang, Ping-Du Lee, Jong-Ching Su |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Starch phosphorylase biology Starch fungi Organic Chemistry food and beverages General Medicine Polysaccharide Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Tissue culture Glycogen phosphorylase chemistry.chemical_compound chemistry Callus biology.protein Kinetin Amylase Molecular Biology Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 57:1311-1315 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.57.1311 |
| Popis: | By controlling the concentrations of kinetin, auxin, and sucrose in the Murashige–Skoog medium, starch contents in callus culture induced from sweet potato tissues could be manipulated. Activity staining and Western analysis on PAGE plates and activity assays made on starch phosphorylase in the presence and absence of mercuric ions showed that β-amylase is absent in callus cultures regardless of whether their starch content is high or low. This would imply that β-amylase induction in sweet potato calli is not linked to the metabolic control through which the expression of storage function is associated, as proposed by Nakamura et al. [Plant Physiol., 96, 902 (1991)] for sweet potato leaf-petiole cuttings. Analyses of starch phosphorylase in crude extracts suggested the presence of a new starch phosphorylase in tuberous root and callus tissue. This phosphorylase is immunologically different from the tuberous root and leaf enzymes that we studied previously. |
| Databáze: | OpenAIRE |
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